Semen analysis is done to study the fertility status of a male.

Collection of Semen:

Sexual abstinance for 5 days.

Semen should be collected in a clean dry container having no detergent.

Not to be collected in condom.

Is to be collected by masturbation or by coitus interruptus.

Laboratory Examination:

The following features are to be noted:

1. Amount: Nornal- at least 0.5 ml in one ejaculation.

2. Colour: White in colour.

3. Consistency: Turbid & Viscid.

4. Total spermatozoa: 20-150 million/ml.

5. Method: Three parts of 5% formalin & one part of saturated gention violet in alcohol is used to stain spermatozoa. Distilled water is tinged with this stain.

Semen is diluted 1 in 10 with this water in WBC pipette. A counting chamber is filled. The number of spermatozoae in 5 small squares (as done in RBC count) are counted. Total spermatozoae is obtained by multiplying the number by 10,00,000.

Motility of spermatozoa: Normal: 40-100%.

Method: A cover slip preparation is made as done for the motility test of bacteria and examined under high power of microscope.

Morphology: Normal: 75-100%.

Method: A film is prepared on a clean glass slide and stained with the stain mentioned under total count. Morphology of head neck and tail are recorded.

Opinion:

nNormozoospermia: If the sperm count is more than 20 million/ml.

nOligospermia: If the sperm count is less than 20 million/ml and have abnormal morphology.

nAzoospermia: If spermatozoa is absent in the semen.

nHyperzoospermia: If the sperm count is more than 150 million/ml. 

PREGNANCY TEST: http://pregnancytestofawomen.blogspot.com/